› 4glw

X-ray diffraction
2Å resolution

Function and Biology Details

Reaction catalysed: 
(1a) NAD(+) + [DNA ligase]-L-lysine = [DNA ligase]-N(6)-(5'-adenylyl)-L-lysine + beta-nicotinamide D-nucleotide
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-111076 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
DNA ligase Chains: A, B
Molecule details ›
Chains: A, B
Length: 305 amino acids
Theoretical weight: 34.59 KDa
Source organism: Streptococcus pneumoniae P1031
Expression system: Escherichia coli
UniProt:
  • Canonical: C1CKI0 (Residues: 1-305; Coverage: 47%)
Gene names: SPP_1122, ligA
Sequence domains: NAD-dependent DNA ligase adenylation domain
Structure domains: DNA ligase/mRNA capping enzyme

Ligands and Environments

3 bound ligands:
Ligand SO4 2 x SO4
Ligand 0XT 2 x 0XT
Ligand NMN 1 x NMN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06DA
Spacegroup: P31
Unit cell:
a: 56.73Å b: 56.73Å c: 197.93Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.209 0.207 0.26
Expression system: Escherichia coli

Quick links

Citations

3 review citations

DNA replication proteins as potential targets for antimicrobials in drug-resistant bacterial pathogens.
van Eijk et al. (2017)
2 more

2 mentions without citation

Copaifera of the Neotropics: A Review of the Phytochemistry and Pharmacology.
da Trindade et al. (2018)
1 more