› 4gaw

X-ray diffraction
3Å resolution

Crystal structure of active human granzyme H

Released:
DOI: 10.2210/pdb4gaw/pdb
PDB entry 4gaw coloured by chain, front view.
PDB entry 4gaw coloured by chain, side view.
PDB entry 4gaw coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Identification of SERPINB1 as a physiological inhibitor of human granzyme H.
Wang L, Li Q, Wu L, Liu S, Zhang Y, Yang X, Zhu P, Zhang H, Zhang K, Lou J, Liu P, Tong L, Sun F, Fan Z
J Immunol 190 1319-30 (2013)
PMID: 23269243

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-149044 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Granzyme H Chains: A, B, C, D, E, F, G, H, I, J, K, L
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I, J, K, L
Length: 226 amino acids
Theoretical weight: 25.21 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P20718 (Residues: 21-246; Coverage: 99%)
Gene names: CGL2, CTSGL2, GZMH
Sequence domains: Trypsin
Structure domains: Trypsin-like serine proteases

Ligands and Environments

2 bound ligands:
Ligand SO4 15 x SO4
Ligand CL 3 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-5A
Spacegroup: P21212
Unit cell:
a: 170.369Å b: 367.07Å c: 61.137Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.272 0.271 0.306
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Granzyme M: behind enemy lines.
de Poot et al. (2014)
2 more