› 4fuw

X-ray diffraction
2.6Å resolution

Crystal structure of Ego3 mutant

Released:
DOI: 10.2210/pdb4fuw/pdb
PDB entry 4fuw coloured by chain, front view.
PDB entry 4fuw coloured by chain, side view.
PDB entry 4fuw coloured by chain, top view.
Source organism: Saccharomyces cerevisiae S288C
Primary publication:
Ego3 functions as a homodimer to mediate the interaction between Gtr1-Gtr2 and Ego1 in the ego complex to activate TORC1.
Zhang T, Péli-Gulli MP, Yang H, De Virgilio C, Ding J
Structure 20 2151-60 (2012)
PMID: 23123112

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-153589 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Protein SLM4 Chains: A, B
Molecule details ›
Chains: A, B
Length: 161 amino acids
Theoretical weight: 18.37 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli
UniProt:
  • Canonical: P38247 (Residues: 1-162; Coverage: 94%)
Gene names: EGO3, GSE1, SLM4, YBR0723, YBR077C
Sequence domains: Protein SLM4
Structure domains: Dynein light chain 2a, cytoplasmic

Ligands and Environments

1 bound ligand:
Ligand SO4 4 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: P43
Unit cell:
a: 60.526Å b: 60.526Å c: 116.851Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.206 0.203 0.253
Expression system: Escherichia coli

Quick links

Citations

19 review citations

mTOR Signaling in Growth, Metabolism, and Disease.
Saxton et al. (2017)
18 more