› 4fis

X-ray diffraction
2.3Å resolution

THE MOLECULAR STRUCTURE OF WILD-TYPE AND A MUTANT FIS PROTEIN: RELATIONSHIP BETWEEN MUTATIONAL CHANGES AND RECOMBINATIONAL ENHANCER FUNCTION OR DNA BINDING

Released:
DOI: 10.2210/pdb4fis/pdb
PDB entry 4fis coloured by chain, front view.
PDB entry 4fis coloured by chain, side view.
PDB entry 4fis coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
The molecular structure of wild-type and a mutant Fis protein: relationship between mutational changes and recombinational enhancer function or DNA binding.
Yuan HS, Finkel SE, Feng JA, Kaczor-Grzeskowiak M, Johnson RC, Dickerson RE
Proc Natl Acad Sci U S A 88 9558-62 (1991)
PMID: 1946369

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-141419 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
DNA-binding protein Fis Chains: A, B
Molecule details ›
Chains: A, B
Length: 98 amino acids
Theoretical weight: 11.2 KDa
Source organism: Escherichia coli
Expression system: Not provided
UniProt:
  • Canonical: P0A6R3 (Residues: 1-98; Coverage: 100%)
Gene names: JW3229, b3261, fis
Sequence domains: Bacterial regulatory protein, Fis family
Structure domains: Homeodomain-like

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: P212121
Unit cell:
a: 79.3Å b: 50.5Å c: 47.1Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.185 0.185 not available
Expression system: Not provided

Quick links

Citations

12 review citations

Control of rRNA transcription in Escherichia coli.
Condon et al. (1995)
11 more

1 mention without citation

Crystal structure of the nucleoid-associated protein Fis (PA4853) from Pseudomonas aeruginosa.
Zhou et al. (2020)