Function and Biology Details
Reaction catalysed:
L-glutamate + H(2)O + NADP(+) = 2-oxoglutarate + NH(3) + NADPH
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
- WD40 repeat
- Glutamate/phenylalanine/leucine/valine/L-tryptophan dehydrogenase
- Glutamate/phenylalanine/leucine/valine/L-tryptophan dehydrogenase, C-terminal
- Nucleoporin Nup120/160
- WD40-repeat-containing domain superfamily
- Nucleoporin Nup37
- WD40/YVTN repeat-like-containing domain superfamily
- NAD(P)-binding domain superfamily
5 more domains
Structure analysis Details
Assemblies composition:
Assembly name:
PDBe Complex ID:
PDB-CPX-128733 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Uncharacterized WD repeat-containing protein C4F10.18
Molecule details ›
Chains: A, C
Length: 394 amino acids
Theoretical weight: 43.05 KDa
Source organism: Schizosaccharomyces pombe 972h-
Expression system: Escherichia coli BL21(DE3)
UniProt:
Sequence domains: WD domain, G-beta repeat
Length: 394 amino acids
Theoretical weight: 43.05 KDa
Source organism: Schizosaccharomyces pombe 972h-
Expression system: Escherichia coli BL21(DE3)
UniProt:
- Canonical:
O36030 (Residues: 1-391; Coverage: 100%)
Sequence domains: WD domain, G-beta repeat
Nucleoporin nup120
Molecule details ›
Chains: B, D
Length: 1139 amino acids
Theoretical weight: 130.14 KDa
Source organism: Schizosaccharomyces pombe 972h-
Expression system: Escherichia coli BL21(DE3)
UniProt:
Sequence domains:
Length: 1139 amino acids
Theoretical weight: 130.14 KDa
Source organism: Schizosaccharomyces pombe 972h-
Expression system: Escherichia coli BL21(DE3)
UniProt:
- Canonical: O43044 (Residues: 1-1136; Coverage: 100%)
Sequence domains:
Glutamate dehydrogenase
Molecule details ›
Chain: X
Length: 450 amino acids
Theoretical weight: 48.86 KDa
Source organism: Escherichia coli O157:H7
Expression system: Escherichia coli BL21(DE3)
UniProt:
Sequence domains:
Length: 450 amino acids
Theoretical weight: 48.86 KDa
Source organism: Escherichia coli O157:H7
Expression system: Escherichia coli BL21(DE3)
UniProt:
- Canonical: Q8XDW9 (Residues: 1-447; Coverage: 100%)
Sequence domains:
Ligands and Environments
No bound ligands
No modified residues
Experiments and Validation Details
X-ray source:
APS BEAMLINE 24-ID-C
Spacegroup:
P6322
Expression system: Escherichia coli BL21(DE3)
