› 4fhb

X-ray diffraction
2.8Å resolution

Enhancing DHFR catalysis by binding of an allosteric regulator nanobody (Nb179)

Released:
DOI: 10.2210/pdb4fhb/pdb
PDB entry 4fhb coloured by chain, front view.
PDB entry 4fhb coloured by chain, side view.
PDB entry 4fhb coloured by chain, top view.
Source organisms:
Primary publication:
Mechanistic analysis of allosteric and non-allosteric effects arising from nanobody binding to two epitopes of the dihydrofolate reductase of Escherichia coli.
Oyen D, Wechselberger R, Srinivasan V, Steyaert J, Barlow JN
Biochim Biophys Acta 1834 2147-57 (2013)
PMID: 23911607

Function and Biology Details

Reaction catalysed: 
5,6,7,8-tetrahydrofolate + NADP(+) = 7,8-dihydrofolate + NADPH
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-209582 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Dihydrofolate reductase Chain: A
Molecule details ›
Chain: A
Length: 159 amino acids
Theoretical weight: 18.02 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P0ABQ4 (Residues: 1-159; Coverage: 100%)
Gene names: JW0047, b0048, folA, tmrA
Sequence domains: Dihydrofolate reductase
Structure domains: Dihydrofolate Reductase, subunit A
Nb179 Chain: D
Molecule details ›
Chain: D
Length: 135 amino acids
Theoretical weight: 14.88 KDa
Source organism: Lama glama
Expression system: Escherichia coli
Structure domains: Immunoglobulins

Ligands and Environments


Cofactor: Ligand NAP 1 x NAP
1 bound ligand:
Ligand FOL 1 x FOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06DA
Spacegroup: P6122
Unit cell:
a: 72.889Å b: 72.889Å c: 254.273Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.221 0.217 0.296
Expression system: Escherichia coli

Quick links

Citations

4 review citations

Camelid nanobodies: killing two birds with one stone.
Desmyter et al. (2015)
3 more

3 mentions without citation

Detection and manipulation of live antigen-expressing cells using conditionally stable nanobodies.
Tang et al. (2016)
2 more