› 4ffb

X-ray diffraction
2.88Å resolution

A TOG:alpha/beta-tubulin Complex Structure Reveals Conformation-Based Mechanisms For a Microtubule Polymerase

Released:
DOI: 10.2210/pdb4ffb/pdb
PDB entry 4ffb coloured by chain, front view.
PDB entry 4ffb coloured by chain, side view.
PDB entry 4ffb coloured by chain, top view.
Source organism: Saccharomyces cerevisiae S288C
Primary publication:
A TOG:αβ-tubulin complex structure reveals conformation-based mechanisms for a microtubule polymerase.
Ayaz P, Ye X, Huddleston P, Brautigam CA, Rice LM
Science 337 857-60 (2012)
PMID: 22904013

Function and Biology Details

Reaction catalysed: 
GTP + H(2)O = GDP + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-135932 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Tubulin alpha-1 chain Chain: A
Molecule details ›
Chain: A
Length: 447 amino acids
Theoretical weight: 49.85 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: P09733 (Residues: 1-447; Coverage: 100%)
Gene names: TUB1, YML085C
Sequence domains:
Structure domains:
Tubulin beta chain Chain: B
Molecule details ›
Chain: B
Length: 463 amino acids
Theoretical weight: 51.91 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: P02557 (Residues: 1-457; Coverage: 100%)
Gene names: TUB2, YFL037W
Sequence domains:
Structure domains:
Protein STU2 Chain: C
Molecule details ›
Chain: C
Length: 278 amino acids
Theoretical weight: 31.67 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P46675 (Residues: 1-272; Coverage: 31%)
Gene names: L2108, STU2, YLR045C
Sequence domains: XMAP215/Dis1/CLASP, TOG domain
Structure domains: Leucine-rich Repeat Variant

Ligands and Environments

2 bound ligands:
Ligand MG 2 x MG
Ligand GTP 2 x GTP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P21
Unit cell:
a: 89.12Å b: 98.04Å c: 91.366Å
α: 90° β: 100.31° γ: 90°
R-values:
R R work R free
0.21 0.208 0.267
Expression systems:
  • Saccharomyces cerevisiae
  • Escherichia coli BL21(DE3)

Quick links

Citations

29 review citations

Control of microtubule organization and dynamics: two ends in the limelight.
Akhmanova et al. (2015)
28 more

33 mentions without citation

The contribution of αβ-tubulin curvature to microtubule dynamics.
Brouhard et al. (2014)
32 more