› 4fe3

X-ray diffraction
1.74Å resolution

Structure of murine cytosolic 5'-nucleotidase III complexed with uridinine monophosphate

Released:
DOI: 10.2210/pdb4fe3/pdb
PDB entry 4fe3 coloured by chain, front view.
PDB entry 4fe3 coloured by chain, side view.
PDB entry 4fe3 coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
Structural basis of substrate specificity and selectivity of murine cytosolic 5'-nucleotidase III.
Grobosky CL, Lopez JB, Rennie S, Skopelitis DJ, Wiest AT, Bingman CA, Bitto E
J Mol Biol 423 540-54 (2012)
PMID: 22925580

Function and Biology Details

Reactions catalysed: 
A 5'-ribonucleotide + H(2)O = a ribonucleoside + phosphate
CMP + H(2)O = cytidine + phosphate
Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-190032 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cytosolic 5'-nucleotidase 3A Chain: A
Molecule details ›
Chain: A
Length: 297 amino acids
Theoretical weight: 33.83 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9D020 (Residues: 46-331; Coverage: 86%)
Gene names: Nt5c3, Nt5c3a
Sequence domains: Pyrimidine 5'-nucleotidase (UMPH-1)
Structure domains:

Ligands and Environments

4 bound ligands:
Ligand BME 2 x BME
Ligand NA 1 x NA
Ligand MG 1 x MG
Ligand U5P 1 x U5P
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-D
Spacegroup: P4322
Unit cell:
a: 46.878Å b: 46.878Å c: 287.101Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.172 0.169 0.218
Expression system: Escherichia coli

Quick links

Citations

1 citations in other articles

Identification of Drosophila and human 7-methyl GMP-specific nucleotidases.
Buschmann et al. (2013)

2 mentions without citation

Crystal structures of the novel cytosolic 5'-nucleotidase IIIB explain its preference for m7GMP.
Monecke et al. (2014)
1 more