› 4f8d

X-ray diffraction
1.5Å resolution

Crystal Structure of an R46A mutant of the Restriction-Modification Controller Protein C.Esp1396I (Monoclinic Form)

Released:
DOI: 10.2210/pdb4f8d/pdb
PDB entry 4f8d coloured by chain, front view.
PDB entry 4f8d coloured by chain, side view.
PDB entry 4f8d coloured by chain, top view.
Source organism: Enterobacter sp. RFL1396
Primary publication:
Structural and mutagenic analysis of the RM controller protein C.Esp1396I.
Martin RN, McGeehan JE, Kneale G
PLoS One 9 e98365 (2014)
PMID: 24887147

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-184509 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
HTH cro/C1-type domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 82 amino acids
Theoretical weight: 9.44 KDa
Source organism: Enterobacter sp. RFL1396
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q8GGH0 (Residues: 1-79; Coverage: 100%)
Gene name: esp1396IC
Sequence domains: Helix-turn-helix
Structure domains: lambda repressor-like DNA-binding domains

Ligands and Environments

2 bound ligands:
Ligand SO4 4 x SO4
Ligand NA 2 x NA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I02
Spacegroup: C2
Unit cell:
a: 46.4Å b: 51Å c: 74.6Å
α: 90° β: 96.6° γ: 90°
R-values:
R R work R free
0.178 0.177 0.2
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 citations in other articles

Temporal dynamics of methyltransferase and restriction endonuclease accumulation in individual cells after introducing a restriction-modification system.
Morozova et al. (2016)

1 mention without citation

Structural and mutagenic analysis of the RM controller protein C.Esp1396I.
Martin et al. (2014)