› 4f4o

X-ray diffraction
2.9Å resolution

Structure of the Haptoglobin-Haemoglobin Complex

Released:
DOI: 10.2210/pdb4f4o/pdb
PDB entry 4f4o coloured by chain, front view.
PDB entry 4f4o coloured by chain, side view.
PDB entry 4f4o coloured by chain, top view.
Source organism: Sus scrofa
Primary publication:
Structure of the haptoglobin-haemoglobin complex.
Andersen CB, Torvund-Jensen M, Nielsen MJ, de Oliveira CL, Hersleth HP, Andersen NH, Pedersen JS, Andersen GR, Moestrup SK
Nature 489 456-9 (2012)
PMID: 22922649

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
PDBe Complex ID:
PDB-CPX-135381 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (5 distinct):
Hemoglobin subunit alpha Chains: A, D, G, J
Molecule details ›
Chains: A, D, G, J
Length: 141 amino acids
Theoretical weight: 15.06 KDa
Source organism: Sus scrofa
UniProt:
  • Canonical: P01965 (Residues: 1-141; Coverage: 100%)
Gene name: HBA
Sequence domains: Globin
Structure domains: Globins
Hemoglobin subunit beta Chains: B, E, H, K
Molecule details ›
Chains: B, E, H, K
Length: 146 amino acids
Theoretical weight: 16.06 KDa
Source organism: Sus scrofa
UniProt:
  • Canonical: P02067 (Residues: 2-147; Coverage: 99%)
Gene name: HBB
Sequence domains: Globin
Structure domains: Globins
Haptoglobin Chains: C, F, I, L
Molecule details ›
Chains: C, F, I, L
Length: 347 amino acids
Theoretical weight: 38.53 KDa
Source organism: Sus scrofa
UniProt:
  • Canonical: Q8SPS7 (Residues: 1-347; Coverage: 100%)
Gene name: HP
Sequence domains: Trypsin
Structure domains:

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, FUC
Carbohydrate polymer
3 bound ligands:
Ligand HEM 8 x HEM
Ligand OXY 8 x OXY
Ligand NAG 8 x NAG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: P212121
Unit cell:
a: 72.88Å b: 197.78Å c: 322.07Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.212 0.211 0.228

Quick links

Citations

31 review citations

Hemolysis and free hemoglobin revisited: exploring hemoglobin and hemin scavengers as a novel class of therapeutic proteins.
Schaer et al. (2013)
30 more

8 mentions without citation

Heme Scavenging and Delivery: The Role of Human Serum Albumin.
De Simone et al. (2023)
7 more