› 4f4j

X-ray diffraction
2.45Å resolution

Conversion of the enzyme guanylate kinase into a mitotic spindle orienting protein by a single mutation that inhibits gmp- induced closing

Released:
DOI: 10.2210/pdb4f4j/pdb
PDB entry 4f4j coloured by chain, front view.
PDB entry 4f4j coloured by chain, side view.
PDB entry 4f4j coloured by chain, top view.
Source organism: Saccharomyces cerevisiae S288C
Primary publication:
Conversion of the enzyme guanylate kinase into a mitotic-spindle orienting protein by a single mutation that inhibits GMP-induced closing.
Johnston CA, Whitney DS, Volkman BF, Doe CQ, Prehoda KE
Proc Natl Acad Sci U S A 108 E973-8 (2011)
PMID: 21990344

Function and Biology Details

Reaction catalysed: 
ATP + GMP = ADP + GDP
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-147375 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Guanylate kinase Chains: A, B
Molecule details ›
Chains: A, B
Length: 202 amino acids
Theoretical weight: 22.42 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli
UniProt:
  • Canonical: P15454 (Residues: 1-187; Coverage: 100%)
Gene names: D9461.39, GUK1, YDR454C
Sequence domains: Guanylate kinase
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand SO4 5 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.1
Spacegroup: P43212
Unit cell:
a: 103.682Å b: 103.682Å c: 130.881Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.219 0.216 0.255
Expression system: Escherichia coli

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Citations

4 review citations

Allosteric activation transitions in enzymes and biomolecular motors: insights from atomistic and coarse-grained simulations.
Daily et al. (2013)
3 more

2 mentions without citation

Evolution of an ancient protein function involved in organized multicellularity in animals.
Anderson et al. (2016)
1 more