› 4f1t

X-ray diffraction
2.3Å resolution

Crystal Structure of the Roco4 Kinase Domain from D. discoideum bound to the ROCK Inhibitor H1152

Released:
DOI: 10.2210/pdb4f1t/pdb
PDB entry 4f1t coloured by chain, front view.
PDB entry 4f1t coloured by chain, side view.
PDB entry 4f1t coloured by chain, top view.
Source organism: Dictyostelium discoideum
Primary publication:
Roco kinase structures give insights into the mechanism of Parkinson disease-related leucine-rich-repeat kinase 2 mutations.
Gilsbach BK, Ho FY, Vetter IR, van Haastert PJ, Wittinghofer A, Kortholt A
Proc Natl Acad Sci U S A 109 10322-7 (2012)
PMID: 22689969

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-180528 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Probable serine/threonine-protein kinase roco4 Chain: A
Molecule details ›
Chain: A
Length: 287 amino acids
Theoretical weight: 32.62 KDa
Source organism: Dictyostelium discoideum
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q6XHB2 (Residues: 1019-1292; Coverage: 16%)
Gene names: DDB_G0288251, roco4
Sequence domains: Protein tyrosine and serine/threonine kinase
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand H52 2 x H52
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X10SA
Spacegroup: P43212
Unit cell:
a: 43.28Å b: 43.28Å c: 352.52Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.199 0.197 0.234
Expression system: Escherichia coli BL21(DE3)

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Citations

17 review citations

LRRK2: cause, risk, and mechanism.
Paisán-Ruiz et al. (2013)
16 more

3 mentions without citation

Roco kinase structures give insights into the mechanism of Parkinson disease-related leucine-rich-repeat kinase 2 mutations.
Gilsbach et al. (2012)
2 more