› 4evo

X-ray diffraction
1.95Å resolution

Crystal structure of Staphylococcal nuclease variant NVIAGA/E122G at cryogenic temperature

Released:
DOI: 10.2210/pdb4evo/pdb
PDB entry 4evo coloured by chain, front view.
PDB entry 4evo coloured by chain, side view.
PDB entry 4evo coloured by chain, top view.
Source organism: Staphylococcus aureus
Entry authors: Doctrow BM, Schlessman JL, Garcia-Moreno E B, Heroux A

Function and Biology Details

Reaction catalysed: 
Endonucleolytic cleavage to nucleoside 3'-phosphates and 3'-phosphooligonucleotide end-products
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132790 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Nuclease A Chain: A
Molecule details ›
Chain: A
Length: 149 amino acids
Theoretical weight: 16.71 KDa
Source organism: Staphylococcus aureus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P00644 (Residues: 83-231; Coverage: 73%)
Gene name: nuc
Sequence domains: Staphylococcal nuclease homologue
Structure domains: OB fold (Dihydrolipoamide Acetyltransferase, E2P)

Ligands and Environments

1 bound ligand:
Ligand PO4 1 x PO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X25
Spacegroup: P41
Unit cell:
a: 48.745Å b: 48.745Å c: 63.224Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.189 0.184 0.227
Expression system: Escherichia coli BL21(DE3)

Quick links