› 4es4

X-ray diffraction
2.9Å resolution

Crystal structure of YdiV and FlhD complex

Released:
DOI: 10.2210/pdb4es4/pdb
PDB entry 4es4 coloured by chain, front view.
PDB entry 4es4 coloured by chain, side view.
PDB entry 4es4 coloured by chain, top view.
Source organism: Escherichia coli K-12
Primary publication:
Structural insight of a concentration-dependent mechanism by which YdiV inhibits Escherichia coli flagellum biogenesis and motility.
Li B, Li N, Wang F, Guo L, Huang Y, Liu X, Wei T, Zhu D, Liu C, Pan H, Xu S, Wang HW, Gu L
Nucleic Acids Res 40 11073-85 (2012)
PMID: 23002140

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-141738 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Putative anti-FlhC(2)FlhD(4) factor YdiV Chains: A, C, E, G
Molecule details ›
Chains: A, C, E, G
Length: 237 amino acids
Theoretical weight: 27.07 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P76204 (Residues: 1-237; Coverage: 100%)
Gene names: JW1697, b1707, cdgR, ydiV
Structure domains: EAL domain
Flagellar transcriptional regulator FlhD Chains: B, D, F, H
Molecule details ›
Chains: B, D, F, H
Length: 116 amino acids
Theoretical weight: 13.33 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P0A8S9 (Residues: 1-116; Coverage: 100%)
Gene names: JW1881, b1892, flbB, flhD
Sequence domains: Flagellar transcriptional activator (FlhD)
Structure domains: Flagellar transcriptional activator FlhD

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: P3121
Unit cell:
a: 132.486Å b: 132.486Å c: 145.685Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.247 0.244 0.283
Expression system: Escherichia coli

Quick links

Citations

4 review citations

Cyclic di-GMP: the first 25 years of a universal bacterial second messenger.
Römling et al. (2013)
3 more

1 mention without citation

Structural insight of a concentration-dependent mechanism by which YdiV inhibits Escherichia coli flagellum biogenesis and motility.
Li et al. (2012)