› 4ei2

X-ray diffraction
3.11Å resolution

Crystal Structures of MthK RCK gating ring bound to Barium

Released:
DOI: 10.2210/pdb4ei2/pdb
PDB entry 4ei2 coloured by chain, front view.
PDB entry 4ei2 coloured by chain, side view.
PDB entry 4ei2 coloured by chain, top view.
Source organism: Methanothermobacter thermautotrophicus str. Delta H
Primary publication:
Crystal structure of a Ba(2+)-bound gating ring reveals elementary steps in RCK domain activation.
Smith FJ, Pau VP, Cingolani G, Rothberg BS
Structure 20 2038-47 (2012)
PMID: 23085076

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-127896 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Calcium-gated potassium channel MthK Chains: A, B, C, D, E, F, G, H, I, J, K, L, M, N, O, P
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I, J, K, L, M, N, O, P
Length: 242 amino acids
Theoretical weight: 26.89 KDa
Source organism: Methanothermobacter thermautotrophicus str. Delta H
Expression system: Escherichia coli
UniProt:
  • Canonical: O27564 (Residues: 107-336; Coverage: 69%)
Gene names: MTH_1520, mthK
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand BA 57 x BA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X6A
Spacegroup: P212121
Unit cell:
a: 110.045Å b: 136.419Å c: 498.427Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.209 0.209 0.242
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Understanding the conformational motions of RCK gating rings.
Giraldez et al. (2017)
2 more

1 mention without citation

Crystal structure of a Ba(2+)-bound gating ring reveals elementary steps in RCK domain activation.
Smith et al. (2012)