› 4e7s

X-ray diffraction
2.25Å resolution

Myosin VI D23R I24R R569E (MD) pre-powerstroke state

Released:
DOI: 10.2210/pdb4e7s/pdb
PDB entry 4e7s coloured by chain, front view.
PDB entry 4e7s coloured by chain, side view.
PDB entry 4e7s coloured by chain, top view.
Source organism: Sus scrofa
Primary publication:
Processive steps in the reverse direction require uncoupling of the lead head lever arm of myosin VI.
Ménétrey J, Isabet T, Ropars V, Mukherjea M, Pylypenko O, Liu X, Perez J, Vachette P, Sweeney HL, Houdusse AM
Mol Cell 48 75-86 (2012)
PMID: 22940248

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-123361 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Unconventional myosin-VI Chains: A, B
Molecule details ›
Chains: A, B
Length: 798 amino acids
Theoretical weight: 91.09 KDa
Source organism: Sus scrofa
Expression system: unidentified baculovirus
UniProt:
  • Canonical: F1RQI7 (Residues: 2-789; Coverage: 62%)
Gene name: MYO6
Sequence domains: Myosin head (motor domain)
Structure domains:

Ligands and Environments

5 bound ligands:
Ligand ADP 2 x ADP
Ligand VO4 2 x VO4
Ligand MG 2 x MG
Ligand GOL 6 x GOL
Ligand PO4 1 x PO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SOLEIL BEAMLINE PROXIMA 1
Spacegroup: P212121
Unit cell:
a: 81.947Å b: 135.14Å c: 196.54Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.181 0.179 0.216
Expression system: unidentified baculovirus

Quick links

Citations

2 review citations

Force generation by kinesin and myosin cytoskeletal motor proteins.
Kull et al. (2013)
1 more

1 mention without citation

Processive steps in the reverse direction require uncoupling of the lead head lever arm of myosin VI.
Ménétrey et al. (2012)