› 4e4e

X-ray diffraction
1.88Å resolution

Crystal Structure of the Y34F mutant of Saccharomyces cerevisiae Manganese Superoxide Dismutase

Released:
DOI: 10.2210/pdb4e4e/pdb
PDB entry 4e4e coloured by chain, front view.
PDB entry 4e4e coloured by chain, side view.
PDB entry 4e4e coloured by chain, top view.
Source organism: Saccharomyces cerevisiae
Primary publication:
Six-coordinate manganese(3+) in catalysis by yeast manganese superoxide dismutase.
Sheng Y, Butler Gralla E, Schumacher M, Cascio D, Cabelli DE, Valentine JS
Proc Natl Acad Sci U S A 109 14314-9 (2012)
PMID: 22908245

Function and Biology Details

Reaction catalysed: 
2 superoxide + 2 H(+) = O(2) + H(2)O(2)
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132508 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Superoxide dismutase [Mn], mitochondrial Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 207 amino acids
Theoretical weight: 23.52 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: P00447 (Residues: 27-233; Coverage: 89%)
Gene names: SOD2, YHR008C
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand MN 4 x MN
1 modified residue:
Ligand MLY 64 x MLY

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: P1
Unit cell:
a: 61.139Å b: 63.022Å c: 63.943Å
α: 112.19° β: 106.78° γ: 103.88°
R-values:
R R work R free
0.192 0.19 0.231
Expression system: Saccharomyces cerevisiae

Quick links

Citations

2 review citations

Superoxide dismutases and superoxide reductases.
Sheng et al. (2014)
1 more

3 mentions without citation

The structure-function relationships and physiological roles of MnSOD mutants.
Bonetta Valentino R. (2022)
2 more