› 4dm2

X-ray diffraction
1.95Å resolution

Contribution of disulfide bond toward thermostability in hyperthermostable endocellulase

Released:
DOI: 10.2210/pdb4dm2/pdb
PDB entry 4dm2 coloured by chain, front view.
PDB entry 4dm2 coloured by chain, side view.
PDB entry 4dm2 coloured by chain, top view.
Source organism: Pyrococcus horikoshii OT3
Entry authors: Kim H-W, Ishikawa K

Function and Biology Details

Reaction catalysed: 
Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-129785 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glycoside hydrolase family 5 domain-containing protein Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 377 amino acids
Theoretical weight: 43.3 KDa
Source organism: Pyrococcus horikoshii OT3
Expression system: Escherichia coli
UniProt:
  • Canonical: O58925 (Residues: 34-410; Coverage: 82%)
Gene name: PH1171
Sequence domains: Cellulase (glycosyl hydrolase family 5)
Structure domains: Glycosidases

Ligands and Environments

1 bound ligand:
Ligand GOL 6 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL44XU
Spacegroup: C2
Unit cell:
a: 160.236Å b: 58.675Å c: 138.539Å
α: 90° β: 108.93° γ: 90°
R-values:
R R work R free
0.168 0.166 0.207
Expression system: Escherichia coli

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