› 4dj5

X-ray diffraction
1.8Å resolution

Proteinase K by Langmuir-Blodgett Hanging Drop Method at 1.8A resolution for Unique Water Distribution

Released:
DOI: 10.2210/pdb4dj5/pdb
PDB entry 4dj5 coloured by chain, front view.
PDB entry 4dj5 coloured by chain, side view.
PDB entry 4dj5 coloured by chain, top view.
Source organism: Parengyodontium album
Primary publication:
Unique water distribution of Langmuir-Blodgett versus classical crystals.
Pechkova E, Sivozhelezov V, Belmonte L, Nicolini C
J Struct Biol 180 57-64 (2012)
PMID: 22706161

Function and Biology Details

Reaction catalysed: 
Hydrolysis of keratin, and of other proteins with subtilisin-like specificity. Hydrolyzes peptide amides.
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-139275 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Proteinase K Chain: X
Molecule details ›
Chain: X
Length: 279 amino acids
Theoretical weight: 28.93 KDa
Source organism: Parengyodontium album
Expression system: Not provided
UniProt:
  • Canonical: P06873 (Residues: 106-384; Coverage: 76%)
Gene name: PROK
Sequence domains: Subtilase family
Structure domains: Peptidase S8/S53 domain

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-4
Spacegroup: P43212
Unit cell:
a: 67.95Å b: 67.95Å c: 102.18Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.159 0.156 0.202
Expression system: Not provided

Quick links

Citations

1 citations in other articles

Langmuir-Blodgett nanotemplates for protein crystallography.
Pechkova et al. (2017)

1 mention without citation

Avoidable errors in deposited macromolecular structures: an impediment to efficient data mining.
Dauter et al. (2014)