› 4dgc

X-ray diffraction
2.65Å resolution

TRIMCyp cyclophilin domain from Macaca mulatta: cyclosporin A complex

Released:
DOI: 10.2210/pdb4dgc/pdb
PDB entry 4dgc coloured by chain, front view.
PDB entry 4dgc coloured by chain, side view.
PDB entry 4dgc coloured by chain, top view.
Source organisms:
Primary publication:
Diverse HIV viruses are targeted by a conformationally dynamic antiviral.
Caines ME, Bichel K, Price AJ, McEwan WA, Towers GJ, Willett BJ, Freund SM, James LC
Nat Struct Mol Biol 19 411-6 (2012)
PMID: 22407016

Function and Biology Details

Reaction catalysed: 
Peptidylproline (omega=180) = peptidylproline (omega=0)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-158810 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Peptidyl-prolyl cis-trans isomerase A Chains: A, B, C, D, E
Molecule details ›
Chains: A, B, C, D, E
Length: 165 amino acids
Theoretical weight: 18.02 KDa
Source organism: Macaca mulatta
Expression system: Escherichia coli
UniProt:
  • Canonical: P62940 (Residues: 1-165; Coverage: 100%)
Gene names: CYPA, PPIA
Sequence domains: Cyclophilin type peptidyl-prolyl cis-trans isomerase/CLD
Structure domains: Cyclophilin-like
cyclosporin A Chains: F, G, H, I, J
Molecule details ›
Chains: F, G, H, I, J
Length: 11 amino acids
Theoretical weight: 1.22 KDa
Source organism: Tolypocladium inflatum
Expression system: Not provided

Ligands and Environments

No bound ligands
6 modified residues:
Ligand DAL 5 x DAL
Ligand MLE 20 x MLE
Ligand MVA 5 x MVA
Ligand BMT 5 x BMT
Ligand ABA 5 x ABA
Ligand SAR 5 x SAR

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-1
Spacegroup: P6122
Unit cell:
a: 108.47Å b: 108.47Å c: 423.06Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.184 0.182 0.216
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

7 review citations

Cellular self-defense: how cell-autonomous immunity protects against pathogens.
Randow et al. (2013)
6 more

1 mention without citation

Ligand-Mediated Biofilm Formation via Enhanced Physical Interaction between a Diguanylate Cyclase and Its Receptor.
Giacalone et al. (2018)