› 4df0

X-ray diffraction
1.5Å resolution

Crystal structure of orotidine 5'-monophosphate decarboxylase from Thermoproteus neutrophilus

Released:
DOI: 10.2210/pdb4df0/pdb
PDB entry 4df0 coloured by chain, front view.
PDB entry 4df0 coloured by chain, side view.
PDB entry 4df0 coloured by chain, top view.
Source organism: Pyrobaculum neutrophilum V24Sta
Entry authors: Fedorov AA, Fedorov EV, Desai B, Gerlt JA, Almo SC

Function and Biology Details

Reaction catalysed: 
Orotidine 5'-phosphate = UMP + CO(2)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-109432 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
orotidine-5'-phosphate decarboxylase Chains: A, B
Molecule details ›
Chains: A, B
Length: 202 amino acids
Theoretical weight: 21.95 KDa
Source organism: Pyrobaculum neutrophilum V24Sta
Expression system: Escherichia coli
UniProt:
  • Canonical: B1Y9Q9 (Residues: 1-202; Coverage: 100%)
Gene name: Tneu_0023
Sequence domains: Orotidine 5'-phosphate decarboxylase / HUMPS family
Structure domains: Aldolase class I

Ligands and Environments

3 bound ligands:
Ligand NI 2 x NI
Ligand SO4 2 x SO4
Ligand CL 1 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: I4132
Unit cell:
a: 171.61Å b: 171.61Å c: 171.61Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.207 0.205 0.238
Expression system: Escherichia coli

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