› 4dbc

X-ray diffraction
1.5Å resolution

Substrate Activation in Aspartate Aminotransferase

Released:
DOI: 10.2210/pdb4dbc/pdb
PDB entry 4dbc coloured by chain, front view.
PDB entry 4dbc coloured by chain, side view.
PDB entry 4dbc coloured by chain, top view.
Source organism: Escherichia coli K-12
Primary publication:
Ground-state electronic destabilization via hyperconjugation in aspartate aminotransferase.
Griswold WR, Castro JN, Fisher AJ, Toney MD
J Am Chem Soc 134 8436-8 (2012)
PMID: 22551424

Function and Biology Details

Reaction catalysed: 
L-aspartate + 2-oxoglutarate = oxaloacetate + L-glutamate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132575 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Aspartate aminotransferase Chain: A
Molecule details ›
Chain: A
Length: 396 amino acids
Theoretical weight: 43.56 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli K-12
UniProt:
  • Canonical: P00509 (Residues: 1-396; Coverage: 100%)
Gene names: JW0911, aspC, b0928
Sequence domains: Aminotransferase class I and II
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand SO4 4 x SO4
Ligand EDO 1 x EDO
Ligand 3QP 1 x 3QP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL9-2
Spacegroup: C2221
Unit cell:
a: 84.29Å b: 155.25Å c: 77.96Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.17 0.169 0.194
Expression system: Escherichia coli K-12

Quick links

Citations

3 review citations

Aspartate aminotransferase: an old dog teaches new tricks.
Toney MD. (2014)
2 more

2 mentions without citation

Ground-state electronic destabilization via hyperconjugation in aspartate aminotransferase.
Griswold et al. (2012)
1 more