› 4d8j

X-ray diffraction
3.55Å resolution

Structure of E. coli MatP-mats complex

Released:
DOI: 10.2210/pdb4d8j/pdb
PDB entry 4d8j coloured by chain, front view.
PDB entry 4d8j coloured by chain, side view.
PDB entry 4d8j coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Molecular basis for a protein-mediated DNA-bridging mechanism that functions in condensation of the E. coli chromosome.
Dupaigne P, Tonthat NK, Espéli O, Whitfill T, Boccard F, Schumacher MA
Mol Cell 48 560-71 (2012)
PMID: 23084832

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero octamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-117305 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Macrodomain Ter protein Chains: A, B, C, D, G, H, K, L
Molecule details ›
Chains: A, B, C, D, G, H, K, L
Length: 150 amino acids
Theoretical weight: 17.73 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0A8N0 (Residues: 1-150; Coverage: 100%)
Gene names: JW0939, b0956, matP, ycbG
Sequence domains:
Structure domains:
5'-D(*TP*TP*CP*GP*TP*GP*AP*CP*AP*TP*TP*GP*TP*CP*AP*CP*GP*AP*A)-3' Chains: F, J, N, P
Molecule details ›
Chains: F, J, N, P
Length: 19 nucleotides
Theoretical weight: 5.82 KDa
Source organism: Escherichia coli
Expression system: Not provided
5'-D(*TP*TP*CP*GP*TP*GP*AP*CP*AP*AP*TP*GP*TP*CP*AP*CP*GP*AP*A)-3' Chains: E, I, M, O
Molecule details ›
Chains: E, I, M, O
Length: 19 nucleotides
Theoretical weight: 5.83 KDa
Source organism: Escherichia coli
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.3.1
Spacegroup: P212121
Unit cell:
a: 114.87Å b: 180.1Å c: 184.95Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.299 0.299 0.313
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

17 review citations

Genome architecture and global gene regulation in bacteria: making progress towards a unified model?
Dorman CJ. (2013)
16 more

2 mentions without citation

Bacterial chromosome organization and segregation.
Badrinarayanan et al. (2015)
1 more