› 4d2h

X-ray diffraction
1.9Å resolution

Crystal structure of the tetramerisation domain of human CtIP

Released:
DOI: 10.2210/pdb4d2h/pdb
PDB entry 4d2h coloured by chain, front view.
PDB entry 4d2h coloured by chain, side view.
PDB entry 4d2h coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
CtIP tetramer assembly is required for DNA-end resection and repair.
Davies OR, Forment JV, Sun M, Belotserkovskaya R, Coates J, Galanty Y, Demir M, Morton CR, Rzechorzek NJ, Jackson SP, Pellegrini L
Nat Struct Mol Biol 22 150-157 (2015)
PMID: 25558984

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-189253 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
DNA endonuclease RBBP8 Chains: A, B, C, D, E, F, G, H
Molecule details ›
Chains: A, B, C, D, E, F, G, H
Length: 38 amino acids
Theoretical weight: 4.51 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q99708 (Residues: 18-52; Coverage: 4%)
Gene names: CTIP, RBBP8

Ligands and Environments

1 bound ligand:
Ligand SO4 14 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SOLEIL BEAMLINE PROXIMA 1
Spacegroup: P21
Unit cell:
a: 35.97Å b: 37.91Å c: 97.79Å
α: 90° β: 90.02° γ: 90°
R-values:
R R work R free
0.214 0.212 0.251
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

9 review citations

Mechanism and regulation of DNA end resection in eukaryotes.
Symington LS. (2016)
8 more

4 mentions without citation

CtIP/Ctp1/Sae2, molecular form fit for function.
Andres et al. (2017)
3 more