› 4d1q

X-ray diffraction
3.4Å resolution

Hermes transposase bound to its terminal inverted repeat

Released:
DOI: 10.2210/pdb4d1q/pdb
PDB entry 4d1q coloured by chain, front view.
PDB entry 4d1q coloured by chain, side view.
PDB entry 4d1q coloured by chain, top view.
Source organism: Musca domestica
Primary publication:
Structural basis of hAT transposon end recognition by Hermes, an octameric DNA transposase from Musca domestica.
Hickman AB, Ewis HE, Li X, Knapp JA, Laver T, Doss AL, Tolun G, Steven AC, Grishaev A, Bax A, Atkinson PW, Craig NL, Dyda F
Cell 158 353-367 (2014)
PMID: 25036632

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero 24-mer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-117302 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
BED-type domain-containing protein Chains: A, B, G, H
Molecule details ›
Chains: A, B, G, H
Length: 536 amino acids
Theoretical weight: 61.32 KDa
Source organism: Musca domestica
Expression system: Escherichia coli
UniProt:
  • Canonical: Q25442 (Residues: 79-612; Coverage: 87%)
Sequence domains:
Structure domains: Zinc finger, BED domain-containing
TERMINAL INVERTED REPEAT Chains: C, E, I, K
Molecule details ›
Chains: C, E, I, K
Length: 15 nucleotides
Theoretical weight: 4.61 KDa
Source organism: Musca domestica
Expression system: Not provided
TERMINAL INVERTED REPEAT Chains: D, F, J, L
Molecule details ›
Chains: D, F, J, L
Length: 16 nucleotides
Theoretical weight: 4.89 KDa
Source organism: Musca domestica
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand NA 4 x NA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007 HF
Spacegroup: P3221
Unit cell:
a: 265.15Å b: 265.15Å c: 157.64Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.212 0.212 0.254
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

4 review citations

Retroviral DNA Integration.
Lesbats et al. (2016)
3 more

7 mentions without citation

Mechanisms of DNA Transposition.
Hickman et al. (2015)
6 more