› 4cla

X-ray diffraction
2Å resolution

ALTERNATIVE BINDING MODES FOR CHLORAMPHENICOL AND 1-SUBSTITUTED CHLORAMPHENICOL ANALOGUES REVEALED BY SITE-DIRECTED MUTAGENESIS AND X-RAY CRYSTALLOGRAPHY OF CHLORAMPHENICOL ACETYLTRANSFERASE

Released:
DOI: 10.2210/pdb4cla/pdb
PDB entry 4cla coloured by chain, front view.
PDB entry 4cla coloured by chain, side view.
PDB entry 4cla coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Alternative binding modes for chloramphenicol and 1-substituted chloramphenicol analogues revealed by site-directed mutagenesis and X-ray crystallography of chloramphenicol acetyltransferase.
Murray IA, Lewendon A, Williams JA, Cullis PM, Shaw WV, Leslie AG
Biochemistry 30 3763-70 (1991)
PMID: 2015231

Function and Biology Details

Reaction catalysed: 
Acetyl-CoA + chloramphenicol = CoA + chloramphenicol 3-acetate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assemblies composition:
homo trimer (preferred)
homo hexamer
Assembly name:
PDBe Complex ID:
PDB-CPX-132547 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Chloramphenicol acetyltransferase 3 Chain: A
Molecule details ›
Chain: A
Length: 213 amino acids
Theoretical weight: 25.06 KDa
Source organism: Escherichia coli
Expression system: Not provided
UniProt:
  • Canonical: P00484 (Residues: 1-213; Coverage: 100%)
Gene name: cat3
Sequence domains: Chloramphenicol acetyltransferase
Structure domains: Chloramphenicol acetyltransferase-like domain

Ligands and Environments

2 bound ligands:
Ligand CO 2 x CO
Ligand CLM 1 x CLM
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: R32
Unit cell:
a: 107.75Å b: 107.75Å c: 123.92Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.157 0.157 not available
Expression system: Not provided

Quick links

Citations

1 review citation

O-Acetyltransferases for chloramphenicol and other natural products.
Murray et al. (1997)
6 other articles

3 mentions without citation

Development of quantitative structure-binding affinity relationship models based on novel geometrical chemical descriptors of the protein-ligand interfaces.
Zhang et al. (2006)
2 more