› 4cbh

X-ray diffraction
2.51Å resolution

Pestivirus NS3 helicase

Released:
DOI: 10.2210/pdb4cbh/pdb
PDB entry 4cbh coloured by chain, front view.
PDB entry 4cbh coloured by chain, side view.
PDB entry 4cbh coloured by chain, top view.
Source organism: Classical swine fever virus
Primary publication:
X-ray structure of the pestivirus NS3 helicase and its conformation in solution.
Tortorici MA, Duquerroy S, Kwok J, Vonrhein C, Perez J, Lamp B, Bricogne G, Rümenapf T, Vachette P, Rey FA
J Virol 89 4356-71 (2015)
PMID: 25653438

Function and Biology Details

Reactions catalysed: 
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1)
Leu is conserved at position P1 for all four cleavage sites. Alanine is found at position P1' of the NS4A-NS4B cleavage site, whereas serine is found at position P1' of the NS3-NS4A, NS4B-NS5A and NS5A-NS5B cleavage sites
NTP + H(2)O = NDP + phosphate
ATP + H(2)O = ADP + phosphate
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-148697 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Serine protease NS3 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 516 amino acids
Theoretical weight: 58.02 KDa
Source organism: Classical swine fever virus
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P19712 (Residues: 1782-2280; Coverage: 13%)
Sequence domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: P21
Unit cell:
a: 70.07Å b: 144.44Å c: 118.69Å
α: 90° β: 92.94° γ: 90°
R-values:
R R work R free
0.205 0.203 0.232
Expression system: Escherichia coli BL21

Quick links

Citations

9 citation in other articles

A positive-strand RNA virus uses alternative protein-protein interactions within a viral protease/cofactor complex to switch between RNA replication and virion morphogenesis.
Dubrau et al. (2017)
8 more

2 mentions without citation

The Eukaryotic Replisome Goes Under the Microscope.
O'Donnell et al. (2016)
1 more