› 4c2t

X-ray diffraction
4Å resolution

Crystal structure of full length Deinococcus radiodurans UvrD in complex with DNA

Released:
DOI: 10.2210/pdb4c2t/pdb
PDB entry 4c2t coloured by chain, front view.
PDB entry 4c2t coloured by chain, side view.
PDB entry 4c2t coloured by chain, top view.
Source organisms:
Primary publication:
Structural and mechanistic insight into DNA unwinding by Deinococcus radiodurans UvrD.
Stelter M, Acajjaoui S, McSweeney S, Timmins J
PLoS One 8 e77364 (2013)
PMID: 24143224

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-117273 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
DNA 3'-5' helicase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 745 amino acids
Theoretical weight: 82.71 KDa
Source organism: Deinococcus radiodurans
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9RTI9 (Residues: 1-745; Coverage: 100%)
Gene name: DR_1775
Sequence domains:
Structure domains:
DNA STRAND FOR28 Chains: M, P
Molecule details ›
Chains: M, P
Length: 28 nucleotides
Theoretical weight: 8.57 KDa
Source organism: synthetic construct
Expression system: Not provided
DNA STRAND REV28 Chains: N, O
Molecule details ›
Chains: N, O
Length: 28 nucleotides
Theoretical weight: 8.58 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

2 bound ligands:
Ligand MG 4 x MG
Ligand ANP 4 x ANP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-1
Spacegroup: P21
Unit cell:
a: 71.457Å b: 390.044Å c: 71.559Å
α: 90° β: 106.13° γ: 90°
R-values:
R R work R free
0.248 0.246 0.271
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

4 review citations

Grip it and rip it: structural mechanisms of DNA helicase substrate binding and unwinding.
Bhattacharyya et al. (2014)
3 more

6 mentions without citation

Structural features and functional implications of proteins enabling the robustness of Deinococcus radiodurans.
Chen et al. (2020)
5 more