› 4c29

X-ray diffraction
2.2Å resolution

Crystal Structure of High-Affinity von Willebrand Factor A1 domain with Disulfide Mutation

Released:
DOI: 10.2210/pdb4c29/pdb
PDB entry 4c29 coloured by chain, front view.
PDB entry 4c29 coloured by chain, side view.
PDB entry 4c29 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural basis of regulation of von Willebrand factor binding to glycoprotein Ib.
Blenner MA, Dong X, Springer TA
J Biol Chem 289 5565-79 (2014)
PMID: 24391089

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-137563 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
von Willebrand factor Chains: A, B
Molecule details ›
Chains: A, B
Length: 215 amino acids
Theoretical weight: 24.71 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P04275 (Residues: 1264-1471; Coverage: 8%)
Gene names: F8VWF, VWF
Sequence domains: von Willebrand factor type A domain
Structure domains: von Willebrand factor, type A domain

Ligands and Environments

3 bound ligands:
Ligand ACT 1 x ACT
Ligand PEG 4 x PEG
Ligand CA 3 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID
Spacegroup: P21
Unit cell:
a: 41.249Å b: 50.071Å c: 96.869Å
α: 90° β: 92.73° γ: 90°
R-values:
R R work R free
0.188 0.186 0.235
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

8 review citations

von Willebrand factor, Jedi knight of the bloodstream.
Springer TA. (2014)
7 more

3 mentions without citation

Structural basis of regulation of von Willebrand factor binding to glycoprotein Ib.
Blenner et al. (2014)
2 more