› 4bts

X-ray diffraction
3.7Å resolution

THE CRYSTAL STRUCTURE OF THE EUKARYOTIC 40S RIBOSOMAL SUBUNIT IN COMPLEX WITH EIF1 AND EIF1A

Released:
DOI: 10.2210/pdb4bts/pdb
PDB entry 4bts coloured by chain, front view.
PDB entry 4bts coloured by chain, side view.
PDB entry 4bts coloured by chain, top view.
Source organisms:
Primary publication:
The crystal structure of the eukaryotic 40S ribosomal subunit in complex with eIF1 and eIF1A.
Weisser M, Voigts-Hoffmann F, Rabl J, Leibundgut M, Ban N
Nat Struct Mol Biol 20 1015-7 (2013)
PMID: 23851459

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero 143-mer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-123030 (preferred)
Entry contents:
35 distinct polypeptide molecules
1 distinct RNA molecule
Macromolecules (36 distinct):
Eukaryotic translation initiation factor 4C Chains: A0, B0, C0, D0
Molecule details ›
Chains: A0, B0, C0, D0
Length: 211 amino acids
Theoretical weight: 24.71 KDa
Source organism: Tetrahymena thermophila SB210
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: I7MK25 (Residues: 2-193; Coverage: 100%)
Gene name: TTHERM_00497990
Sequence domains: Translation initiation factor 1A / IF-1
40S ribosomal protein rpS28e Chains: A1, B1, C1, D1
Molecule details ›
Chains: A1, B1, C1, D1
Length: 68 amino acids
Theoretical weight: 7.63 KDa
Source organism: Tetrahymena thermophila
UniProt:
  • Canonical: E6PBU6 (Residues: 1-68; Coverage: 100%)
Sequence domains: Ribosomal protein S28e
40S ribosomal protein S8 Chains: A2, B2, C2, D2
Molecule details ›
Chains: A2, B2, C2, D2
Length: 208 amino acids
Theoretical weight: 24.25 KDa
Source organism: Tetrahymena thermophila
UniProt:
  • Canonical: E6PBS6 (Residues: 1-208; Coverage: 100%)
Sequence domains: Ribosomal protein S8e
40S ribosomal protein S7 Chains: A3, B3, C3, D3
Molecule details ›
Chains: A3, B3, C3, D3
Length: 197 amino acids
Theoretical weight: 23.23 KDa
Source organism: Tetrahymena thermophila
UniProt:
  • Canonical: E6PBS5 (Residues: 1-197; Coverage: 100%)
Sequence domains: Ribosomal protein S7e
Small ribosomal subunit protein eS1 Chains: A4, B4, C4, D4
Molecule details ›
Chains: A4, B4, C4, D4
Length: 265 amino acids
Theoretical weight: 29.71 KDa
Source organism: Tetrahymena thermophila
UniProt:
  • Canonical: E6PBR8 (Residues: 1-265; Coverage: 100%)
Sequence domains: Ribosomal S3Ae family
40S ribosomal protein S26 Chains: A5, B5, C5, D5
Molecule details ›
Chains: A5, B5, C5, D5
Length: 119 amino acids
Theoretical weight: 13.84 KDa
Source organism: Tetrahymena thermophila
UniProt:
  • Canonical: E6PBU4 (Residues: 1-119; Coverage: 100%)
Sequence domains: Ribosomal protein S26e
40S ribosomal protein S27 Chains: A6, B6, C6, D6
Molecule details ›
Chains: A6, B6, C6, D6
Length: 81 amino acids
Theoretical weight: 9.25 KDa
Source organism: Tetrahymena thermophila
UniProt:
  • Canonical: E6PBU5 (Residues: 1-81; Coverage: 100%)
Sequence domains: Ribosomal protein S27
Plectin/eS10 N-terminal domain-containing protein Chains: A7, B7, C7, D7
Molecule details ›
Chains: A7, B7, C7, D7
Length: 162 amino acids
Theoretical weight: 18.61 KDa
Source organism: Tetrahymena thermophila
UniProt:
  • Canonical: E6PBS8 (Residues: 1-162; Coverage: 100%)
Sequence domains: Plectin/S10 domain
40S ribosomal protein S25 Chains: A8, B8, C8, D8
Molecule details ›
Chains: A8, B8, C8, D8
Length: 143 amino acids
Theoretical weight: 15.3 KDa
Source organism: Tetrahymena thermophila
UniProt:
  • Canonical: E6PBU3 (Residues: 1-143; Coverage: 100%)
Sequence domains: S25 ribosomal protein
Ubiquitin-like domain-containing protein Chains: A9, B9, C9, D9
Molecule details ›
Chains: A9, B9, C9, D9
Length: 189 amino acids
Theoretical weight: 20.9 KDa
Source organism: Tetrahymena thermophila
UniProt:
  • Canonical: E6PBU9 (Residues: 1-189; Coverage: 100%)
Sequence domains: Ribosomal protein S27a
Small ribosomal subunit protein uS2 Chains: AB, BB, CB, DB
Molecule details ›
Chains: AB, BB, CB, DB
Length: 241 amino acids
Theoretical weight: 27.53 KDa
Source organism: Tetrahymena thermophila
UniProt:
  • Canonical: E6PBR9 (Residues: 1-241; Coverage: 100%)
Sequence domains: Ribosomal protein S2
40S ribosomal protein S3 Chains: AC, BC, CC, DC
Molecule details ›
Chains: AC, BC, CC, DC
Length: 243 amino acids
Theoretical weight: 27.58 KDa
Source organism: Tetrahymena thermophila
UniProt:
  • Canonical: E6PBS1 (Residues: 1-243; Coverage: 100%)
Sequence domains:
Small ribosomal subunit protein uS4 N-terminal domain-containing protein Chains: AD, BD, CD, DD
Molecule details ›
Chains: AD, BD, CD, DD
Length: 181 amino acids
Theoretical weight: 21.19 KDa
Source organism: Tetrahymena thermophila
UniProt:
  • Canonical: E6PBS7 (Residues: 1-181; Coverage: 100%)
Sequence domains:
S5 DRBM domain-containing protein Chains: AE, BE, CE, DE
Molecule details ›
Chains: AE, BE, CE, DE
Length: 296 amino acids
Theoretical weight: 33.74 KDa
Source organism: Tetrahymena thermophila
UniProt:
  • Canonical: E6PBS0 (Residues: 1-296; Coverage: 100%)
Sequence domains:
SUI1 domain-containing protein Chains: AF, BF, CF, DF
Molecule details ›
Chains: AF, BF, CF, DF
Length: 101 amino acids
Theoretical weight: 11.96 KDa
Source organism: Tetrahymena thermophila
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: E6PBV1 (Residues: 1-101; Coverage: 100%)
Sequence domains: Translation initiation factor SUI1
Small ribosomal subunit protein uS7 domain-containing protein Chains: AG, BG, CG, DG
Molecule details ›
Chains: AG, BG, CG, DG
Length: 200 amino acids
Theoretical weight: 22.51 KDa
Source organism: Tetrahymena thermophila
UniProt:
  • Canonical: E6PBS3 (Residues: 1-200; Coverage: 100%)
Sequence domains: Ribosomal protein S7p/S5e
40S ribosomal protein rpS22e Chains: AH, BH, CH, DH
Molecule details ›
Chains: AH, BH, CH, DH
Length: 130 amino acids
Theoretical weight: 14.93 KDa
Source organism: Tetrahymena thermophila
UniProt:
  • Canonical: E6PBU0 (Residues: 1-130; Coverage: 100%)
Sequence domains: Ribosomal protein S8
Rps16e Chains: AI, BI, CI, DI
Molecule details ›
Chains: AI, BI, CI, DI
Length: 145 amino acids
Theoretical weight: 16.42 KDa
Source organism: Tetrahymena thermophila
UniProt:
  • Canonical: E6PBT4 (Residues: 1-145; Coverage: 100%)
Sequence domains: Ribosomal protein S9/S16
Small ribosomal subunit protein uS10 domain-containing protein Chains: AJ, BJ, CJ, DJ
Molecule details ›
Chains: AJ, BJ, CJ, DJ
Length: 120 amino acids
Theoretical weight: 13.7 KDa
Source organism: Tetrahymena thermophila
UniProt:
  • Canonical: E6PBT8 (Residues: 1-120; Coverage: 100%)
Sequence domains: Ribosomal protein S10p/S20e
40S ribosomal protein rpS14e Chains: AK, BK, CK, DK
Molecule details ›
Chains: AK, BK, CK, DK
Length: 151 amino acids
Theoretical weight: 16.4 KDa
Source organism: Tetrahymena thermophila
UniProt:
  • Canonical: E6PBT2 (Residues: 1-151; Coverage: 100%)
Sequence domains: Ribosomal protein S11
Small ribosomal subunit protein uS12 Chains: AL, BL, CL, DL
Molecule details ›
Chains: AL, BL, CL, DL
Length: 142 amino acids
Theoretical weight: 15.78 KDa
Source organism: Tetrahymena thermophila
UniProt:
  • Canonical: P06147 (Residues: 1-142; Coverage: 100%)
Sequence domains: Ribosomal protein S12/S23
Rps18e Chains: AM, BM, CM, DM
Molecule details ›
Chains: AM, BM, CM, DM
Length: 155 amino acids
Theoretical weight: 17.8 KDa
Source organism: Tetrahymena thermophila
UniProt:
  • Canonical: E6PBT6 (Residues: 1-155; Coverage: 100%)
Sequence domains: Ribosomal protein S13/S18
40S ribosomal protein rpS29e Chains: AN, BN, CN, DN
Molecule details ›
Chains: AN, BN, CN, DN
Length: 55 amino acids
Theoretical weight: 6.66 KDa
Source organism: Tetrahymena thermophila
UniProt:
  • Canonical: E6PBU7 (Residues: 1-55; Coverage: 100%)
Sequence domains: Ribosomal protein S14p/S29e
Small ribosomal subunit protein uS15 N-terminal domain-containing protein Chains: AO, BO, CO, DO
Molecule details ›
Chains: AO, BO, CO, DO
Length: 153 amino acids
Theoretical weight: 17.62 KDa
Source organism: Tetrahymena thermophila
UniProt:
  • Canonical: E6PBT1 (Residues: 1-153; Coverage: 100%)
Sequence domains:
40S ribosomal protein S24 Chains: AP, BP, CP, DP
Molecule details ›
Chains: AP, BP, CP, DP
Length: 149 amino acids
Theoretical weight: 17.13 KDa
Source organism: Tetrahymena thermophila
UniProt:
  • Canonical: E6PBU2 (Residues: 1-149; Coverage: 100%)
Sequence domains: Ribosomal protein S24e
Small ribosomal subunit protein uS17 N-terminal domain-containing protein Chains: AQ, BQ, CQ, DQ
Molecule details ›
Chains: AQ, BQ, CQ, DQ
Length: 157 amino acids
Theoretical weight: 18.14 KDa
Source organism: Tetrahymena thermophila
UniProt:
  • Canonical: E6PBS9 (Residues: 1-157; Coverage: 100%)
Sequence domains:
40S ribosomal protein RACK1 Chains: AR, BR, CR, DR
Molecule details ›
Chains: AR, BR, CR, DR
Length: 343 amino acids
Theoretical weight: 38.52 KDa
Source organism: Tetrahymena thermophila
UniProt:
  • Canonical: E6PBV0 (Residues: 1-343; Coverage: 100%)
Sequence domains: WD domain, G-beta repeat
Rps15e Chains: AS, BS, CS, DS
Molecule details ›
Chains: AS, BS, CS, DS
Length: 144 amino acids
Theoretical weight: 15.91 KDa
Source organism: Tetrahymena thermophila
UniProt:
  • Canonical: E6PBT3 (Residues: 1-144; Coverage: 100%)
Sequence domains: Ribosomal protein S19
40S ribosomal protein rpS19e Chains: AT, BT, CT, DT
Molecule details ›
Chains: AT, BT, CT, DT
Length: 155 amino acids
Theoretical weight: 17.77 KDa
Source organism: Tetrahymena thermophila
UniProt:
  • Canonical: E6PBT7 (Residues: 1-155; Coverage: 100%)
Sequence domains: Ribosomal protein S19e
Ribosomal protein eL8/eL30/eS12/Gadd45 domain-containing protein Chains: AU, BU, CU, DU
Molecule details ›
Chains: AU, BU, CU, DU
Length: 126 amino acids
Theoretical weight: 13.83 KDa
Source organism: Tetrahymena thermophila
UniProt:
  • Canonical: E6PBT0 (Residues: 1-126; Coverage: 100%)
Sequence domains: Ribosomal protein L7Ae/L30e/S12e/Gadd45 family
40S ribosomal protein rpS17e Chains: AV, BV, CV, DV
Molecule details ›
Chains: AV, BV, CV, DV
Length: 130 amino acids
Theoretical weight: 14.96 KDa
Source organism: Tetrahymena thermophila
UniProt:
  • Canonical: E6PBT5 (Residues: 1-130; Coverage: 100%)
Sequence domains: Ribosomal S17
40S ribosomal protein S4 Chains: AW, BW, CW, DW
Molecule details ›
Chains: AW, BW, CW, DW
Length: 259 amino acids
Theoretical weight: 29.51 KDa
Source organism: Tetrahymena thermophila
UniProt:
  • Canonical: E6PBS2 (Residues: 2-260; Coverage: 100%)
Sequence domains:
40S ribosomal protein S30 Chains: AX, BX, CX, DX
Molecule details ›
Chains: AX, BX, CX, DX
Length: 80 amino acids
Theoretical weight: 9.25 KDa
Source organism: Tetrahymena thermophila
UniProt:
  • Canonical: E6PBU8 (Residues: 1-80; Coverage: 100%)
Sequence domains: Ribosomal protein S30
40S ribosomal protein rpS6e Chains: AY, BY, CY, DY
Molecule details ›
Chains: AY, BY, CY, DY
Length: 293 amino acids
Theoretical weight: 32.61 KDa
Source organism: Tetrahymena thermophila
UniProt:
  • Canonical: E6PBS4 (Residues: 1-293; Coverage: 100%)
Sequence domains: Ribosomal protein S6e
40S ribosomal protein rpS21e Chains: AZ, BZ, CZ, DZ
Molecule details ›
Chains: AZ, BZ, CZ, DZ
Length: 97 amino acids
Theoretical weight: 10.7 KDa
Source organism: Tetrahymena thermophila
UniProt:
  • Canonical: E6PBT9 (Residues: 1-97; Coverage: 100%)
Sequence domains: Ribosomal protein S21e
18S Ribosomal RNA Chains: AA, BA, CA, DA
Molecule details ›
Chains: AA, BA, CA, DA
Length: 1753 nucleotides
Theoretical weight: 565.19 KDa
Sequence domains: Eukaryotic small subunit ribosomal RNA

Ligands and Environments

2 bound ligands:
Ligand ZN 16 x ZN
Ligand MG 316 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: P21
Unit cell:
a: 209.99Å b: 471.55Å c: 298.54Å
α: 90° β: 91.02° γ: 90°
R-values:
R R work R free
0.202 0.202 0.229
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

16 review citations

The scanning mechanism of eukaryotic translation initiation.
Hinnebusch AG. (2014)
15 more