› 4bmd

X-ray diffraction
2.5Å resolution

Crystal structure of S.pombe Rad4 BRCT3,4

Released:
DOI: 10.2210/pdb4bmd/pdb
PDB entry 4bmd coloured by chain, front view.
PDB entry 4bmd coloured by chain, side view.
PDB entry 4bmd coloured by chain, top view.
Source organism: Schizosaccharomyces pombe
Primary publication:
Phosphorylation-dependent assembly and coordination of the DNA damage checkpoint apparatus by Rad4(TopBP1).
Qu M, Rappas M, Wardlaw CP, Garcia V, Ren JY, Day M, Carr AM, Oliver AW, Du LL, Pearl LH
Mol Cell 51 723-736 (2013)
PMID: 24074952

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-152284 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
S-M checkpoint control protein rad4 Chain: A
Molecule details ›
Chain: A
Length: 204 amino acids
Theoretical weight: 23.74 KDa
Source organism: Schizosaccharomyces pombe
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P32372 (Residues: 291-494; Coverage: 32%)
Gene names: SPAC23C4.18c, cut5, rad4
Sequence domains: BRCA1 C Terminus (BRCT) domain
Structure domains: BRCT domain

Ligands and Environments

2 bound ligands:
Ligand CL 1 x CL
Ligand MES 1 x MES
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04-1
Spacegroup: P3121
Unit cell:
a: 93.03Å b: 93.03Å c: 75.39Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.186 0.184 0.218
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

4 review citations

TopBP1: A BRCT-scaffold protein functioning in multiple cellular pathways.
Wardlaw et al. (2014)
3 more

1 mention without citation

Phosphorylation-dependent assembly and coordination of the DNA damage checkpoint apparatus by Rad4(TopBP1).
Qu et al. (2013)