4bhp

Solution NMR

A structural model of CAP mutant (T127L and S128I) in cGMP-bound state

Released:
Source organism: Escherichia coli
Primary publication:
Allosteric inhibition through suppression of transient conformational states.
Nat Chem Biol 9 462-5 (2013)
PMID: 23644478

Function and Biology Details

Structure analysis Details

Assembly composition:
monomeric (preferred)
PDBe Complex ID:
PDB-CPX-142282 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
DNA-binding transcriptional dual regulator CRP Chain: A
Molecule details ›
Chain: A
Length: 209 amino acids
Theoretical weight: 23.58 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0ACJ8 (Residues: 2-210; Coverage: 100%)
Gene names: JW5702, b3357, cap, crp, csm
Sequence domains:
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Chemical shift assignment: 12%
Refinement method: NTORSION ANGLE DYNAMICS, SIMULATED SIMULATED
Expression system: Escherichia coli BL21(DE3)