› 4bea

X-ray diffraction
2.57Å resolution

Crystal Structure of eIF4E in Complex with a Stapled Peptide Derivative

Released:
DOI: 10.2210/pdb4bea/pdb
PDB entry 4bea coloured by chain, front view.
PDB entry 4bea coloured by chain, side view.
PDB entry 4bea coloured by chain, top view.
Source organisms:
Primary publication:
Rational optimization of conformational effects induced by hydrocarbon staples in peptides and their binding interfaces.
Lama D, Quah ST, Verma CS, Lakshminarayanan R, Beuerman RW, Lane DP, Brown CJ
Sci Rep 3 3451 (2013)
PMID: 24336354

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-139168 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Eukaryotic translation initiation factor 4E Chain: A
Molecule details ›
Chain: A
Length: 217 amino acids
Theoretical weight: 25.13 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P06730 (Residues: 1-217; Coverage: 100%)
Gene names: EIF4E, EIF4EL1, EIF4F
Sequence domains: Eukaryotic initiation factor 4E
Structure domains: RNA Cap, Translation Initiation Factor Eif4e
STAPLED EIF4E INTERACTING PEPTIDE Chain: B
Molecule details ›
Chain: B
Length: 13 amino acids
Theoretical weight: 1.56 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MK8 2 x MK8

Experiments and Validation Details

Entry percentile scores
X-ray source: BRUKER X8 PROTEUM
Spacegroup: C2221
Unit cell:
a: 38.301Å b: 91.677Å c: 137.908Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.222 0.22 0.266
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

10 review citations

Structure-Based Design of Inhibitors of Protein-Protein Interactions: Mimicking Peptide Binding Epitopes.
Pelay-Gimeno et al. (2015)
9 more

5 mentions without citation

Stapled Peptides Inhibitors: A New Window for Target Drug Discovery.
Ali et al. (2019)
4 more