› 4bcy

X-ray diffraction
1.27Å resolution

Monomeric Human Cu,Zn Superoxide dismutase, mutation H43F

Released:
DOI: 10.2210/pdb4bcy/pdb
PDB entry 4bcy coloured by chain, front view.
PDB entry 4bcy coloured by chain, side view.
PDB entry 4bcy coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Global structural motions from the strain of a single hydrogen bond.
Danielsson J, Awad W, Saraboji K, Kurnik M, Lang L, Leinartaite L, Marklund SL, Logan DT, Oliveberg M
Proc Natl Acad Sci U S A 110 3829-34 (2013)
PMID: 23431167

Function and Biology Details

Reaction catalysed: 
2 superoxide + 2 H(+) = O(2) + H(2)O(2)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132490 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Superoxide dismutase [Cu-Zn] Chain: A
Molecule details ›
Chain: A
Length: 153 amino acids
Theoretical weight: 15.83 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P00441 (Residues: 2-154; Coverage: 99%)
Gene name: SOD1
Sequence domains: Copper/zinc superoxide dismutase (SODC)
Structure domains: Superoxide dismutase, copper/zinc binding domain

Ligands and Environments

4 bound ligands:
Ligand CU 2 x CU
Ligand ZN 1 x ZN
Ligand CD 6 x CD
Ligand CL 2 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: MAX II BEAMLINE I911-2
Spacegroup: P212121
Unit cell:
a: 34.87Å b: 47.91Å c: 81.18Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.192 0.191 0.203
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

3 review citations

Structural Properties and Interaction Partners of Familial ALS-Associated SOD1 Mutants.
Huai et al. (2019)
2 more

2 mentions without citation

Global structural motions from the strain of a single hydrogen bond.
Danielsson et al. (2013)
1 more