› 4b8z

X-ray diffraction
2.75Å resolution

Crystal structure of human GDP-L-fucose synthase with bound NADP and GDP, rhombohedral crystal form

Released:
DOI: 10.2210/pdb4b8z/pdb
PDB entry 4b8z coloured by chain, front view.
PDB entry 4b8z coloured by chain, side view.
PDB entry 4b8z coloured by chain, top view.
Source organism: Homo sapiens
Entry authors: Vollmar M, Krojer T, Shafqat N, Rojkova A, Bunkoczi G, Yue WW, Kavanagh K, von Delft F, Weigelt J, Arrowsmith CH, Bountra C, Edwards A, Oppermann U

Function and Biology Details

Reaction catalysed: 
GDP-beta-L-fucose + NADP(+) = GDP-4-dehydro-alpha-D-rhamnose + NADPH
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-171792 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
GDP-L-fucose synthase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 320 amino acids
Theoretical weight: 36 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q13630 (Residues: 8-320; Coverage: 98%)
Gene names: GFUS, SDR4E1, TSTA3
Sequence domains: NAD dependent epimerase/dehydratase family
Structure domains:

Ligands and Environments


Cofactor: Ligand NAP 4 x NAP
1 bound ligand:
Ligand GDP 4 x GDP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X10SA
Spacegroup: R3
Unit cell:
a: 91.447Å b: 91.447Å c: 429.13Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.235 0.233 0.261
Expression system: Escherichia coli BL21(DE3)

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