› 4b6z

X-ray diffraction
1.9Å resolution

Crystal structure of metallo-carboxypeptidase from Burkholderia cenocepacia

Released:
DOI: 10.2210/pdb4b6z/pdb
PDB entry 4b6z coloured by chain, front view.
PDB entry 4b6z coloured by chain, side view.
PDB entry 4b6z coloured by chain, top view.
Source organism: Burkholderia cenocepacia
Primary publication:
High-resolution structure of the M14-type cytosolic carboxypeptidase from Burkholderia cenocepacia refined exploiting PDB_REDO strategies.
Rimsa V, Eadsforth TC, Joosten RP, Hunter WN
Acta Crystallogr D Biol Crystallogr 70 279-89 (2014)
PMID: 24531462

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-110030 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Peptidase M14 carboxypeptidase A domain-containing protein Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 405 amino acids
Theoretical weight: 45.2 KDa
Source organism: Burkholderia cenocepacia
Expression system: Escherichia coli
UniProt:
  • Canonical: B4EEQ5 (Residues: 1-384; Coverage: 100%)
Gene name: BCAL2317
Sequence domains:
Structure domains:

Ligands and Environments

6 bound ligands:
Ligand ZN 4 x ZN
Ligand GOL 9 x GOL
Ligand ACT 4 x ACT
Ligand EDO 7 x EDO
Ligand CL 3 x CL
Ligand PEG 1 x PEG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-2
Spacegroup: P212121
Unit cell:
a: 62.897Å b: 85.947Å c: 289.016Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.166 0.164 0.205
Expression system: Escherichia coli

Quick links

Citations

1 citations in other articles

An assessment of three human methylenetetrahydrofolate dehydrogenase/cyclohydrolase-ligand complexes following further refinement.
Bueno et al. (2019)

2 mentions without citation

Loss of tubulin deglutamylase CCP1 causes infantile-onset neurodegeneration.
Shashi et al. (2018)
1 more