› 4b3v

X-ray diffraction
1.98Å resolution

Crystal structure of the Rubella virus glycoprotein E1 in its post-fusion form crystallized in presence of 20mM of Calcium Acetate

Released:
DOI: 10.2210/pdb4b3v/pdb
PDB entry 4b3v coloured by chain, front view.
PDB entry 4b3v coloured by chain, side view.
PDB entry 4b3v coloured by chain, top view.
Source organism: Rubella virus
Primary publication:
Functional and evolutionary insight from the crystal structure of rubella virus protein E1.
DuBois RM, Vaney MC, Tortorici MA, Kurdi RA, Barba-Spaeth G, Krey T, Rey FA
Nature 493 552-6 (2013)
PMID: 23292515

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-140176 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Spike glycoprotein E1 Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 473 amino acids
Theoretical weight: 50.57 KDa
Source organism: Rubella virus
Expression system: Drosophila melanogaster
UniProt:
  • Canonical: P08563 (Residues: 583-1018; Coverage: 41%)
Sequence domains: Rubella membrane glycoprotein E1
Structure domains:

Ligands and Environments

8 bound ligands:
Ligand NAG 4 x NAG
Ligand NGA 2 x NGA
Ligand GOL 15 x GOL
Ligand ACT 7 x ACT
Ligand PG4 1 x PG4
Ligand PEG 4 x PEG
Ligand NA 1 x NA
Ligand CA 3 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SOLEIL BEAMLINE PROXIMA 1
Spacegroup: P212121
Unit cell:
a: 121.272Å b: 126.163Å c: 129.375Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.185 0.185 0.198
Expression system: Drosophila melanogaster

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Citations

18 review citations

Viral membrane fusion.
Harrison SC. (2015)
17 more

3 mentions without citation

Mechanistic Insight into Bunyavirus-Induced Membrane Fusion from Structure-Function Analyses of the Hantavirus Envelope Glycoprotein Gc.
Guardado-Calvo et al. (2016)
2 more