› 4b3f

X-ray diffraction
2.5Å resolution

crystal structure of Ighmbp2 helicase

Released:
DOI: 10.2210/pdb4b3f/pdb
PDB entry 4b3f coloured by chain, front view.
PDB entry 4b3f coloured by chain, side view.
PDB entry 4b3f coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
The Ighmbp2 helicase structure reveals the molecular basis for disease-causing mutations in DMSA1.
Lim SC, Bowler MW, Lai TF, Song H
Nucleic Acids Res 40 11009-22 (2012)
PMID: 22965130

Function and Biology Details

Reaction catalysed: 
ATP + H(2)O = ADP + phosphate
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-153775 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
DNA-binding protein SMUBP-2 Chain: X
Molecule details ›
Chain: X
Length: 646 amino acids
Theoretical weight: 71.63 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P38935 (Residues: 3-648; Coverage: 65%)
Gene names: IGHMBP2, SMBP2, SMUBP2
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand PO4 1 x PO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF, NSRRC BEAMLINE BL13B1
Spacegroup: C2
Unit cell:
a: 116.566Å b: 76.717Å c: 88.536Å
α: 90° β: 107.33° γ: 90°
R-values:
R R work R free
0.193 0.19 0.252
Expression system: Escherichia coli

Quick links

Citations

5 review citations

Clinical and molecular features and therapeutic perspectives of spinal muscular atrophy with respiratory distress type 1.
Vanoli et al. (2015)
4 more

6 mentions without citation

The Ighmbp2 helicase structure reveals the molecular basis for disease-causing mutations in DMSA1.
Lim et al. (2012)
5 more