› 4az6

X-ray diffraction
1.36Å resolution

Differential inhibition of the tandem GH20 catalytic modules in the pneumococcal exo-beta-D-N-acetylglucosaminidase, StrH

Released:
DOI: 10.2210/pdb4az6/pdb
PDB entry 4az6 coloured by chain, front view.
PDB entry 4az6 coloured by chain, side view.
PDB entry 4az6 coloured by chain, top view.
Source organism: Streptococcus pneumoniae TIGR4
Primary publication:
Inhibition of the family 20 glycoside hydrolase catalytic modules in the Streptococcus pneumoniae exo-β-D-N-acetylglucosaminidase, StrH.
Pluvinage B, Stubbs KA, Hattie M, Vocadlo DJ, Boraston AB
Org Biomol Chem 11 7907-15 (2013)
PMID: 24132305

Function and Biology Details

Reaction catalysed: 
Hydrolysis of terminal non-reducing N-acetyl-D-hexosamine residues in N-acetyl-beta-D-hexosaminides
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-155969 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Beta-N-acetylhexosaminidase Chain: A
Molecule details ›
Chain: A
Length: 435 amino acids
Theoretical weight: 48.34 KDa
Source organism: Streptococcus pneumoniae TIGR4
Expression system: Escherichia coli
UniProt:
  • Canonical: P49610 (Residues: 181-613; Coverage: 34%)
Gene names: SP_0057, strH
Sequence domains: Glycosyl hydrolase family 20, catalytic domain
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand OAN 1 x OAN
Ligand EDO 3 x EDO
Ligand P15 1 x P15
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL9-2
Spacegroup: I222
Unit cell:
a: 78.3Å b: 109.6Å c: 112.6Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.116 0.115 0.14
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Glycan-metabolizing enzymes in microbe-host interactions: the Streptococcus pneumoniae paradigm.
Hobbs et al. (2018)
5 other articles