› 4ay2

X-ray diffraction
2.8Å resolution

Capturing 5' tri-phosphorylated RNA duplex by RIG-I

Released:
DOI: 10.2210/pdb4ay2/pdb
PDB entry 4ay2 coloured by chain, front view.
PDB entry 4ay2 coloured by chain, side view.
PDB entry 4ay2 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Visualizing the determinants of viral RNA recognition by innate immune sensor RIG-I.
Luo D, Kohlway A, Vela A, Pyle AM
Structure 20 1983-8 (2012)
PMID: 23022350

Function and Biology Details

Reaction catalysed: 
ATP + H(2)O = ADP + phosphate
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132023 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
Antiviral innate immune response receptor RIG-I Chain: A
Molecule details ›
Chain: A
Length: 687 amino acids
Theoretical weight: 78.69 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: O95786 (Residues: 239-925; Coverage: 74%)
Gene names: DDX58, RIGI
Sequence domains:
Structure domains:
5'-R-PPP(GP*GP*CP*GP*CP*GP*GP*CP*UP*UP*CP*GP*GP*CP *CP*GP*CP*GP*CP*C)-3' Chain: C
Molecule details ›
Chain: C
Length: 20 nucleotides
Theoretical weight: 6.58 KDa
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

3 bound ligands:
Ligand ZN 1 x ZN
Ligand ADP 1 x ADP
Ligand MG 1 x MG
1 modified residue:
Ligand GTP 1 x GTP

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: P212121
Unit cell:
a: 47.745Å b: 76.156Å c: 221.222Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.222 0.218 0.286
Expression system: Not provided

Quick links

Citations

16 review citations

Cytosolic sensing of viruses.
Goubau et al. (2013)
15 more

14 mentions without citation

Parts, assembly and operation of the RIG-I family of motors.
Rawling et al. (2014)
13 more