› 4aju

X-ray diffraction
2.65Å resolution

Crystal structure of the reactive loop cleaved ZPI in P41 space group

Released:
DOI: 10.2210/pdb4aju/pdb
PDB entry 4aju coloured by chain, front view.
PDB entry 4aju coloured by chain, side view.
PDB entry 4aju coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural basis for catalytic activation of protein Z-dependent protease inhibitor (ZPI) by protein Z.
Huang X, Yan Y, Tu Y, Gatti J, Broze GJ, Zhou A, Olson ST
Blood 120 1726-33 (2012)
PMID: 22786881

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-193836 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Protein Z-dependent protease inhibitor Chain: A
Molecule details ›
Chain: A
Length: 387 amino acids
Theoretical weight: 44.36 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9UK55 (Residues: 23-408; Coverage: 91%)
Gene names: SERPINA10, UNQ707/PRO1358, ZPI
Sequence domains: Serpin (serine protease inhibitor)
Structure domains:
Protein Z-dependent protease inhibitor Chain: B
Molecule details ›
Chain: B
Length: 36 amino acids
Theoretical weight: 4.17 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9UK55 (Residues: 409-444; Coverage: 9%)
Gene names: SERPINA10, UNQ707/PRO1358, ZPI

Ligands and Environments

2 bound ligands:
Ligand SO4 4 x SO4
Ligand EDO 1 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I03
Spacegroup: P41
Unit cell:
a: 100.698Å b: 100.698Å c: 78.972Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.218 0.215 0.258
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

2 review citations

Inhibitory serpins. New insights into their folding, polymerization, regulation and clearance.
Gettins et al. (2016)
1 more