› 4age

X-ray diffraction
4.84Å resolution

MTSSL spin labeled D67C mutant of MscS in the open form

Released:
DOI: 10.2210/pdb4age/pdb
PDB entry 4age coloured by chain, front view.
PDB entry 4age coloured by chain, side view.
PDB entry 4age coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Conformational state of the MscS mechanosensitive channel in solution revealed by pulsed electron-electron double resonance (PELDOR) spectroscopy.
Pliotas C, Ward R, Branigan E, Rasmussen A, Hagelueken G, Huang H, Black SS, Booth IR, Schiemann O, Naismith JH
Proc Natl Acad Sci U S A 109 E2675-82 (2012)
PMID: 23012406

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo heptamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-142906 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Small-conductance mechanosensitive channel Chains: A, B, C, D, E, F, G
Molecule details ›
Chains: A, B, C, D, E, F, G
Length: 286 amino acids
Theoretical weight: 30.91 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0C0S1 (Residues: 1-286; Coverage: 100%)
Gene names: JW2891, b2924, mscS, yggB
Sequence domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04
Spacegroup: P212121
Unit cell:
a: 127.07Å b: 149.51Å c: 174.4Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.34 0.339 0.354
Expression system: Escherichia coli

Quick links

Citations

7 review citations

Bacterial mechanosensitive channels: progress towards an understanding of their roles in cell physiology.
Booth IR. (2014)
6 more

5 mentions without citation

MscS-like mechanosensitive channels in plants and microbes.
Wilson et al. (2013)
4 more