4adq

X-ray diffraction
4.5Å resolution

CRYSTAL STRUCTURE OF THE MOUSE COLONY-STIMULATING FACTOR 1 (MCSF-1) CYTOKINE IN COMPLEX WITH THE VIRAL RECEPTOR BARF1

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dodecamer (preferred)
PDBe Complex ID:
PDB-CPX-136665 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (4 distinct):
Secreted protein BARF1 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 208 amino acids
Theoretical weight: 23.41 KDa
Source organism: human gammaherpesvirus 4
Expression system: Homo sapiens
UniProt:
  • Canonical: P03228 (Residues: 21-221; Coverage: 100%)
Gene name: BARF1
Macrophage colony-stimulating factor 1 43 kDa subunit Chains: E, F, G, H
Molecule details ›
Chains: E, F, G, H
Length: 153 amino acids
Theoretical weight: 17.87 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P07141 (Residues: 33-181; Coverage: 29%)
Gene names: Csf1, Csfm
Sequence domains: Macrophage colony stimulating factor-1 (CSF-1)

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, BMA
Carbohydrate polymer : NEW Components: NAG, BMA, MAN
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: R3
Unit cell:
a: 234.75Å b: 234.75Å c: 96.04Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.226 0.223 0.262
Expression systems:
  • Homo sapiens
  • Escherichia coli BL21(DE3)