› 3zuk

X-ray diffraction
2.6Å resolution

CRYSTAL STRUCTURE OF MYCOBACTERIUM TUBERCULOSIS ZINC METALLOPROTEASE ZMP1 IN COMPLEX WITH INHIBITOR

Released:
DOI: 10.2210/pdb3zuk/pdb
PDB entry 3zuk coloured by chain, front view.
PDB entry 3zuk coloured by chain, side view.
PDB entry 3zuk coloured by chain, top view.
Source organism: Mycobacterium tuberculosis H37Rv
Primary publication:
Crystal structure of Mycobacterium tuberculosis zinc-dependent metalloprotease-1 (Zmp1), a metalloprotease involved in pathogenicity.
Ferraris DM, Sbardella D, Petrera A, Marini S, Amstutz B, Coletta M, Sander P, Rizzi M
J Biol Chem 286 32475-82 (2011)
PMID: 21813647

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-129460 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Endopeptidase, peptidase family M13 Chains: A, B
Molecule details ›
Chains: A, B
Length: 699 amino acids
Theoretical weight: 78.13 KDa
Source organism: Mycobacterium tuberculosis H37Rv
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O53649 (Residues: 1-663; Coverage: 100%)
Gene name: MT0208
Sequence domains:
Structure domains:

Ligands and Environments

10 bound ligands:
Ligand ZN 2 x ZN
Ligand RDF 2 x RDF
Ligand 211 1 x 211
Ligand PEG 30 x PEG
Ligand PGE 8 x PGE
Ligand PG4 4 x PG4
Ligand ACT 13 x ACT
Ligand SO4 6 x SO4
Ligand CL 3 x CL
Ligand CA 1 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-1
Spacegroup: P21212
Unit cell:
a: 128.29Å b: 198.77Å c: 63.27Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.177 0.175 0.25
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

3 review citations

Opportunities for Overcoming Mycobacterium tuberculosis Drug Resistance: Emerging Mycobacterial Targets and Host-Directed Therapy.
Torfs et al. (2019)
2 more

7 mentions without citation

Crystal structure of Mycobacterium tuberculosis zinc-dependent metalloprotease-1 (Zmp1), a metalloprotease involved in pathogenicity.
Ferraris et al. (2011)
6 more