› 3zt6

X-ray diffraction
2.19Å resolution

GlgE isoform 1 from Streptomyces coelicolor with alpha-cyclodextrin and maltose bound

Released:
DOI: 10.2210/pdb3zt6/pdb
PDB entry 3zt6 coloured by chain, front view.
PDB entry 3zt6 coloured by chain, side view.
PDB entry 3zt6 coloured by chain, top view.
Source organism: Streptomyces coelicolor
Primary publication:
Structure of Streptomyces maltosyltransferase GlgE, a homologue of a genetically validated anti-tuberculosis target.
Syson K, Stevenson CEM, Rejzek M, Fairhurst SA, Nair A, Bruton CJ, Field RA, Chater KF, Lawson DM, Bornemann S
J Biol Chem 286 38298-38310 (2011)
PMID: 21914799

Function and Biology Details

Reaction catalysed: 
Alpha-maltose 1-phosphate + ((1->4)-alpha-D-glucosyl)(n) = phosphate + ((1->4)-alpha-D-glucosyl)(n+2)
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-192197 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (3 distinct):
Alpha-1,4-glucan:maltose-1-phosphate maltosyltransferase 1 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 695 amino acids
Theoretical weight: 77.56 KDa
Source organism: Streptomyces coelicolor
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9L1K2 (Residues: 1-675; Coverage: 100%)
Gene names: SC6A11.19c, SCO5443, glgE1, pep1, pep1A, pep1I
Sequence domains:
Structure domains:

Ligands and Environments

Carbohydrate polymer : NEW Components: GLC
Carbohydrate polymer
Carbohydrate polymer : NEW Components: GLC
Carbohydrate polymer
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04
Spacegroup: P212121
Unit cell:
a: 113.85Å b: 114.16Å c: 315.61Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.209 0.208 0.236
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

9 review citations

Advances in understanding glycosyltransferases from a structural perspective.
Gloster TM. (2014)
8 more

3 mentions without citation

Structure of Streptomyces maltosyltransferase GlgE, a homologue of a genetically validated anti-tuberculosis target.
Syson et al. (2011)
2 more