› 3zkr

X-ray diffraction
3.65Å resolution

X-ray structure of a pentameric ligand gated ion channel from Erwinia chrysanthemi (ELIC) in complex with bromoform

Released:
DOI: 10.2210/pdb3zkr/pdb
PDB entry 3zkr coloured by chain, front view.
PDB entry 3zkr coloured by chain, side view.
PDB entry 3zkr coloured by chain, top view.
Source organism: Dickeya chrysanthemi
Primary publication:
Multisite binding of a general anesthetic to the prokaryotic pentameric Erwinia chrysanthemi ligand-gated ion channel (ELIC).
Spurny R, Billen B, Howard RJ, Brams M, Debaveye S, Price KL, Weston DA, Strelkov SV, Tytgat J, Bertrand S, Bertrand D, Lummis SCR, Ulens C
J Biol Chem 288 8355-8364 (2013)
PMID: 23364792

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo pentamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-143154 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cys-loop ligand-gated ion channel Chains: A, B, C, D, E, F, G, H, I, J
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I, J
Length: 307 amino acids
Theoretical weight: 35.38 KDa
Source organism: Dickeya chrysanthemi
Expression system: Escherichia coli
UniProt:
  • Canonical: P0C7B7 (Residues: 11-163, 164-316; Coverage: 95%)
Sequence domains: Neurotransmitter-gated ion-channel ligand binding domain
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand MBR 22 x MBR
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06DA
Spacegroup: P21
Unit cell:
a: 105.109Å b: 266.248Å c: 110.746Å
α: 90° β: 109.78° γ: 90°
R-values:
R R work R free
0.23 0.229 0.264
Expression system: Escherichia coli

Quick links

Citations

13 review citations

Structural insights into Cys-loop receptor function and ligand recognition.
Nys et al. (2013)
12 more

10 mentions without citation

Seeking structural specificity: direct modulation of pentameric ligand-gated ion channels by alcohols and general anesthetics.
Howard et al. (2014)
9 more