› 3zkg

X-ray diffraction
1.9Å resolution

Function and Biology Details

Reaction catalysed: 
Broad endopeptidase specificity. Cleaves Glu-Val-Asn-Leu-|-Asp-Ala-Glu-Phe in the Swedish variant of Alzheimer's amyloid precursor protein.
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-195305 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Beta-secretase 2 Chains: A, B
Molecule details ›
Chains: A, B
Length: 386 amino acids
Theoretical weight: 42.05 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9Y5Z0 (Residues: 75-460; Coverage: 78%)
Gene names: AEPLC, ALP56, ASP21, BACE2, CDA13, UNQ418/PRO852
Sequence domains: Eukaryotic aspartyl protease
Structure domains: Acid Proteases

Ligands and Environments

1 bound ligand:
Ligand B3P 1 x B3P
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X10SA
Spacegroup: P21
Unit cell:
a: 46.887Å b: 88.466Å c: 98.572Å
α: 90° β: 96.58° γ: 90°
R-values:
R R work R free
0.231 0.228 0.273
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

5 review citations

Nanobodies as Probes for Protein Dynamics in Vitro and in Cells.
Dmitriev et al. (2016)
4 more

2 mentions without citation

Pepsin-like aspartic proteases (PAPs) as model systems for combining biomolecular simulation with biophysical experiments.
Bhakat S. (2021)
1 more