› 3zjx

X-ray diffraction
2.4Å resolution

Clostridium perfringens epsilon toxin mutant H149A bound to octyl glucoside

Released:
DOI: 10.2210/pdb3zjx/pdb
PDB entry 3zjx coloured by chain, front view.
PDB entry 3zjx coloured by chain, side view.
PDB entry 3zjx coloured by chain, top view.
Source organism: Clostridium perfringens D
Primary publication:
Clostridium perfringens epsilon toxin H149A mutant as a platform for receptor binding studies.
Bokori-Brown M, Kokkinidou MC, Savva CG, Fernandes da Costa S, Naylor CE, Cole AR, Moss DS, Basak AK, Titball RW
Protein Sci 22 650-9 (2013)
PMID: 23504825

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-123058 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Epsilon-toxin Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 289 amino acids
Theoretical weight: 32.08 KDa
Source organism: Clostridium perfringens D
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: E7D8R1 (Residues: 47-305, 306-328; Coverage: 84%)
Gene name: etx
Sequence domains: Clostridium epsilon toxin ETX/Bacillus mosquitocidal toxin MTX2
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand BOG 4 x BOG
Ligand PO4 4 x PO4
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: P3
Unit cell:
a: 123.709Å b: 123.709Å c: 127.314Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.248 0.247 0.275
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

5 review citations

Pore-forming toxins: ancient, but never really out of fashion.
Dal Peraro et al. (2016)
4 more

5 mentions without citation

Diversity and function of arthropod endosymbiont toxins.
Massey et al. (2022)
4 more