› 3zh0

X-ray diffraction
2Å resolution

Functional and structural role of the N-terminal extension in Methanosarcina acetivorans protoglobin

Released:
DOI: 10.2210/pdb3zh0/pdb
PDB entry 3zh0 coloured by chain, front view.
PDB entry 3zh0 coloured by chain, side view.
PDB entry 3zh0 coloured by chain, top view.
Source organism: Methanosarcina acetivorans C2A
Primary publication:
Functional and structural roles of the N-terminal extension in Methanosarcina acetivorans protoglobin.
Ciaccio C, Pesce A, Tundo GR, Tilleman L, Bertolacci L, Dewilde S, Moens L, Ascenzi P, Bolognesi M, Nardini M, Coletta M
Biochim Biophys Acta 1834 1813-23 (2013)
PMID: 23485914

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-186286 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Globin-sensor domain-containing protein Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 176 amino acids
Theoretical weight: 20.87 KDa
Source organism: Methanosarcina acetivorans C2A
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8TLY9 (Residues: 21-195; Coverage: 90%)
Gene name: MA_2883
Sequence domains: Protoglobin
Structure domains: Globins

Ligands and Environments

3 bound ligands:
Ligand HEM 4 x HEM
Ligand FMT 4 x FMT
Ligand GOL 8 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-4
Spacegroup: P21
Unit cell:
a: 80.67Å b: 48.62Å c: 92.03Å
α: 90° β: 92.61° γ: 90°
R-values:
R R work R free
0.202 0.199 0.26
Expression system: Escherichia coli

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Citations

2 citation in other articles

Protoglobin-Catalyzed Formation of cis-Trifluoromethyl-Substituted Cyclopropanes by Carbene Transfer.
Schaus et al. (2023)
1 more