› 3zfh

X-ray diffraction
2.25Å resolution

Crystal structure of Pseudomonas aeruginosa inosine 5'-monophosphate dehydrogenase

Released:
DOI: 10.2210/pdb3zfh/pdb
PDB entry 3zfh coloured by chain, front view.
PDB entry 3zfh coloured by chain, side view.
PDB entry 3zfh coloured by chain, top view.
Source organism: Pseudomonas aeruginosa PAO1
Primary publication:
Structure of Pseudomonas aeruginosa inosine 5'-monophosphate dehydrogenase.
Rao VA, Shepherd SM, Owen R, Hunter WN
Acta Crystallogr Sect F Struct Biol Cryst Commun 69 243-7 (2013)
PMID: 23519796

Function and Biology Details

Reaction catalysed: 
Inosine 5'-phosphate + NAD(+) + H(2)O = xanthosine 5'-phosphate + NADH
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-191112 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Inosine-5'-monophosphate dehydrogenase Chain: A
Molecule details ›
Chain: A
Length: 510 amino acids
Theoretical weight: 54.18 KDa
Source organism: Pseudomonas aeruginosa PAO1
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9HXM5 (Residues: 1-489; Coverage: 100%)
Gene names: PA3770, guaB
Sequence domains:
Structure domains: Aldolase class I

Ligands and Environments

1 bound ligand:
Ligand CL 1 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007
Spacegroup: I4
Unit cell:
a: 115.546Å b: 115.546Å c: 56.443Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.15 0.148 0.19
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

5 citation in other articles

Increased riboflavin production by manipulation of inosine 5'-monophosphate dehydrogenase in Ashbya gossypii.
Buey et al. (2015)
4 more

1 mention without citation

Structure of Pseudomonas aeruginosa inosine 5'-monophosphate dehydrogenase.
Rao et al. (2013)